MORPHOMETRIC CHARACTERISTICS OF THE ADRENAL GLANDS IN NEWBORNS WITH BIRTH-RELATED BRAIN INJURY
Keywords:
adrenal gland injury assessment, morphometric method, birth-related brain injury, necrosis, dystrophy.Abstract
The aim of this study was to comparatively assess the time-dependent morphometric parameters of adrenal gland tissue in newborns who died within the first 24 hours of life (up to 8 hours, up to 16 hours, and up to 24 hours) as a result of birth-related brain injury. The study included adrenal tissue samples obtained during autopsy from preterm newborns who died with a diagnosis of birth-related brain injury. Histological specimens were prepared, and their digital images were obtained using a NanoZoomer histoscanner. Morphometric analysis was performed on digital images of tangential tissue sections. The area of a single field of view was standardized to 32,000 μm², 64,000 μm², and 128,000 μm². Morphometric parameters were measured along the X and Y axes within each predefined field of view, and the obtained data were comparatively analyzed according to the time from birth to death.
The findings of the study allow the identification and assessment of time-dependent morphometric changes in adrenal gland tissue in newborns with birth-related brain injury.
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