ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF POTATO VIRUS F (PVF)

Abstract

This study investigates the isolation, identification, and characterization of Potato Virus F (PVF), an economically important but understudied plant virus affecting potato (Solanum tuberosum L.). Infected plant samples were collected from different agro-ecological zones of Uzbekistan and subjected to morphological and serological examination. Serological detection was carried out using the enzyme-linked immunosorbent assay (ELISA), confirming the presence of PVF in symptomatic plants. Molecular diagnosis was performed using reverse transcription polymerase chain reaction (RT-PCR), which enabled the amplification and partial sequencing of the viral genome. The phylogenetic analysis of PVF isolates revealed genetic divergence from other geographically distinct strains. The biological impact of PVF was also assessed, showing significant reductions in plant growth and tuber yield. Additionally, the study discusses potential pathways of virus transmission, including vegetative propagation and insect vectors, and suggests management strategies to prevent the spread of PVF in potato cultivation.